LABORATORY ANIMALS

Prize

First Prize for the poster presentation entitled: “Experimental model for the study of melanoma. Diagnostic approach with the combined use of acoustic microscopy and infrared spectroscopy, evaluated by histological analysis”. Annual Joint Meeting of the Hellenic Society for Biomedical and Laboratory Animal Science (HSBLAS) in collaboration with the European Society of Laboratory Animal Veterinarians (ESLAV) and the European College of Laboratory Animal Medicine (ECLAM), Athens, Greece; 2014

Seminars

On line course “Recognition of pain, suffering and distress and its application in the evaluation of severity of the procedures (species specific: mice and rats)” Edition 2, provided by “Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise «G. Caporale»”; from 22 October 2020 to 31 December 2020

PATHBIO summer course, Universitat Autònoma de Barcelona, Barcelona, June 2019: Embryology, Anatomy, Histology and the Anatomical Basis of Imaging, a detailed course on mouse. http://www.pathbio.org/summer_course_2019.php

2-11 September 2017: 3^rd LAS (Laboratory Animal Science European Union Functions Course), Athens, Greece: https://hsblas.gr/lasfunctionscourse/

Febr. – March, 2000: Participation in seminars for resident veterinarians from the Division of Laboratory Animal Medicine at UCLA and Scripps Institute, San Diego, CA, USA

9-20, January 1995: Participation in a Seminar: “Laboratory Animals: Use in clinical research and diagnosis-alternative methods”. Organized by the School of Veterinary Medicine in Thessaloniki with the collaboration of the University of Utrecht, the Netherlands, FELASA, and the Branch of Veterinary Medicine of the Agricultural Ministry, Greece

Teaching

Pre-graduate courses

Elective course: Introduction to Laboratory Animal Science at the students of the 5^th semester and higher, School of Veterinary Medicine, Faculty of Health Sciences, AUTh (2017 – present)

Post-graduate courses

Courses at the students of the Master Program: «Principles in experimental studies with animals», School of Medicine, Faculty of Health Sciences, AUTh. Instructror “rodent anatomy” and “factors influencing research” (2018 – present)

Courses at workshops and seminars

Instructor at the courses: “Αnatomy of rodents” at Laboratory Animal Science European Union Functions Course, Athens. (2015-present): https://hsblas.gr/wp-content/uploads/2020/09/Program-2020-Distance-Version-1.pdf

On line presentation at the 1^st LAS Core Modules Course entitled: «Mouse anatomy and physiology» 9-10 July 2020, Alexander Fleming Institute, Athens: https://www.chem.auth.gr/wp-content/uploads/2020/06/1st-LAS-Core-Modules-Course.pdf

Lecture on the “Basic handling on laboratory animals” in the conference organized by the International Veterinary Students Association (IVSA), May 2017, Faculty of Veterinary Medicine, AUTh, University Campus

Presentation entitled: “Severe Combined ImmunoDeficient mice at the biomedical research” Seminars of the Hellenic Society for Biomedical Research and Laboratory Animals, Athens, 2006 and 2008.

Research grant

As Principal Investigator

03/2007-03/2008: “Comparative analysis of histological parameters of normal and neoplasmatic nervous system in Severe Combined ImmunoDeficient (SCID) mice” Alpha Bank

Code of AUTh Research Committee: 82343

As collaborator

15/4/2014-31/8/2014: «In Vivo Preclinical Safety Assessment of Cell Therapy HMS cell administration in Immunodeficient (SCID) mice»: Cell Therapy Limited, UK with P. I.: Maria Tsantarliotou, Ass. Prof. of School of Veterinary Medicine, AUTh, Thessaloniki, Greece, Code of AUTh Research Committee: 91048

2011: «Evaluation of intracellular transport of human recombinant TAT-L-Sco2 protein in animal tissue’ mitochondria in protein therapy of metabolic diseases». AUTh Research Committee with P. I.: Lefkothea Papadopoulou, Associate Professor of School of Pharmacy, AUTh, Thessaloniki, Greece, Code of AUTh Research Committee: 87849

Contribution in systematic review

“A systematic review on the implementation and reporting of refinements in mouse telemetry implantation surgery using electrocardiography (ECG) recording devices”: Alexandra Gkrouzoudi, Anastasia Tsingotjidou & Paulin Jirkof: https://osf.io/rd24w/

Kaifas, D. Papagiannopoulou, A. Miliotou, A. S. Tsingotjidou, P.C. Chalkidou, A. C. Tsika, G.A. Spyroulias, A. S. Tsiftsoglou and L.C. Papadopoulou (2020) In vivo Biodistribution study of TAT-L-Sco2 Fusion Protein, Developed as Protein Therapeutic for Mitochondrial Disorders attributed to SCO2 Mutations, Molecular Genetics and Metabolism Reports, 2020 Dec;25:100683. DOI: 10.1016/j.ymgmr.2020.100683.

Spyridopoulou K, Aindelis G, Lampri E, Giorgalli M, Lamprianidou E, Kotsianidis I, Tsingotjidou A, Pappa A, Kalogirou O, Chlichlia K. (2018). Improving the Subcutaneous Mouse Tumor Model by Effective Manipulation of Magnetic Nanoparticles-Treated Implanted Cancer Cells. Ann Biomed Eng. 2018 Dec;46(12):1975-1987. doi: 10.1007/s10439-018-2107-6. Epub 2018 Aug 3.

Α. S. Τsingotjidou (2009). Severe Combined Immuno Deficient (SCID) mice in biomedical research. Journal of Hellenic Veterinary Medicine Association, 60 (3):266-273 (after invitation of the journal).

Invited speaker/symposium chairing

34^th Medical Conference of North Greece: «Pathologic anatomy examination of rodents», 4– 6, April, 2019, Thessaloniki, Greece.

33^rd Medical Conference of North Greece: «Pathologic anatomy examination of rodents», 22– 24, March, 2018, Thessaloniki, Greece.

32^nd Medical Conference of North Greece: «Anesthesia and blood sampling of rodents», 30, March – 1, April, 2017, Thessaloniki, Greece

Lecture on: «The Science of Laboratory Animals» in the conference organized by the International Veterinary Students Association (IVSA), 29 March 2012, Tsirogiannis Amphitheatre, Faculty of Veterinary Medicine, AUTh, University Campus

Presentations in meetings

Karypidou Marina, Mosialos Konstantinos, Petrellis George, Tsingotjidou Anastasia, Experimental Animal Models of Leishmaniasis and their contribution in the Immunology of the disease. Oral presentation at the 7^th Hellenic Meeting of International Veterinary Students Association (IVSA), 2019

Tsingotjidou Anastasia. (2015). Conditioned Place Preference Test (CPP): use, benefits and pitfalls. The AAALAC International, ESLAV and ECLAM Conference 2015. Hannover – Germany.

Kalman R., Shafrir E., Tsingotjidou A., Ziv E. (2007). The gerbil Psammomys obesus is a model for primary insulin resistance inducing diabetes with implication to humans. 10th FELASA symposium. Cernobbio – Italy.

Tsingotjidou Anastasia (2007). Humanized animal models of cancer metastasis to bone. 10th FELASA symposium. Cernobbio – Italy.

Tsingotjidou A., Kalman R. (2006). Severe Combined ImmunoDeficient (SCID) mice in biomedical research. GV-SOLAS/ESLAV Meeting. Freiburg – Germany.

Meetings’ attendance

2015: AAALAC International, ESLAV and ECLAM Conference, Hannover – Germany

2008: 2^nd Joint Meeting of European Laboratory Animal Veterinarians/LAVA, Dublin, Ireland

2007: 10^th Symposium of Federation of Laboratory Animals, Cernobbio, Italy

2006: GV-SOLAS/European Society for Laboratory Animal Veterinarians, Freiburg, Germany

2005: 1^st East Mediterranean Regional ICLAS Symposium on Laboratory Animal Science, Athens

Member of the Scientific Committee of the Hellenic Society for Biomedical and Laboratory Animal Science (HSBLAS) Meeting in collaboration with the European Society of Laboratory Animal Veterinarians (ESLAV) and the European College of Laboratory Animal Medicine (ECLAM). Athens, September, 2014.

https://hsblas2014.files.wordpress.com/2014/06/joint-hsblas-eslav-eclam-meeting_final-programme.pdf

Member of Scientific Societies

Hellenic Society of Biomedical and Laboratory Animal Science

European Society for Laboratory Animal Veterinarians

LASA The Laboratory Animal Science Association, UK

Member of Administrative Committees

Governing Council of the Hellenic Society for Biomedical Research and Laboratory Animals, 2009 – 2013 – member (elected);

2014 – 2018: vice-president (elected);

2018 – present: President

Participation in Committees, Working Groups and Councils

FELASA WG on Pain Management in Mice, convened by Paul Flecknell (2018-present): http://www.felasa.eu/working-groups/working-groups-present/pain-management-in-mice/

Working group of FELASA (Federation of European Laboratory Animal Science Associations) for the celebration of its 40^th anniversary, 2018-2019

Member of the Board of Management of the Federation of Laboratory Animal Science Associations (FELASA), 2017-present.

Associate member of the National Committee for the Welfare of Animals Used for Scientific Purposes (according to the no. 45/236079/18.9. 2020 decision of the Minister of Rural Development and Food published at the Government Gazette no. 4200 / Vol. Β. / 29.9.2020).

National Liaison of the Hellenic Society of Neurosciences at the CARE Committee (CARE: Committee on Animals in Research), of FENS (Federation of European Neuroscience) and at EARA (European Animal Research Association).

Member of the European Veterinarians in Education, Research and Industry (EVERI), of FVE (Federation of Veterinarians of Europe), 2016-2017.

Participation in the use of laboratory animals for research purposes

Responsible for the construction of the Biocontainment Unit at the Laboratory of Anatomy, Histology and Embryology, where immunodeficient mice can also be housed, 2006-2007. (with the financial support of Alpha Bank for the purchase of the equipment and the completion of the construction by the technical service of the AUTh).

Scientist in charge (facility manager) for the laboratory animals used in the Laboratory of Anatomy, Histology and Embryology for both teaching and research purposes, 2007 – today

More specifically:

for the operation license of the laboratory animal supply installation of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIOsup-47.

Principal Investigator of the Programs with the AUTh Research Committee (code numbers: 94795 and 97838) entitled: “Services for research with the use of laboratory animals”

for the operation license of the laboratory animal breeding installation of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIObr-25.

for the operation license of the laboratory animal experiment installation of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIOexp-24 and EL-54-BIOexp-23.

Designated veterinarian of the laboratory animal installation (for experiments, for breeding and supply) of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIOexp-24, EL-54-BIOexp-23, EL-54-BIOsup-47 and EL-54-BIObr-25 (up to 16-11-2018).

Responsible for compliance with the Presidential Decree 56/2013 of the laboratory animal installation (for experiments, for breeding and supply) of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIOexp-24, EL-54-BIOexp-23, EL-54-BIOsup-47 and EL-54-BIObr-25.

Associate veterinarian of the Protocol Evaluation Committee for the laboratory animal installation (for experiments and breeding) of the Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine with code # EL-54-BIOexp-24, EL-54-BIOexp-23, and EL-54-BIObr-25.

Associate veterinarian of the Protocol Evaluation Committee for the laboratory animal experiment installation of the Laboratory of Experimental Neurology and Neuroimmunology B’ Neurology Clinic AHEPA with code # EL54BIO29.

Member of Organizing Committees

Second meeting of the National Animal Welfare and Ethical Review Body, 1 November 2019, School of Veterinary Medicine, Faculty of Health Sciences, AUTh

Seminars organized by Hellenic Society for Biomedical and Laboratory Animal Science (2018-2020)

Seminar on the introduction of Lab Animal Use, February 2020, Athens
Seminar on the introduction in Lab Animal Technicians, December 2019, Athens
Understanding Animal Research presentation by Bella Williams at the Lab. of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, AUTh, October 2019, Thessaloniki
Seminar on the introduction of Lab Animal Use, February 2019, Athens
Seminar on the introduction in Lab Animal Veterinary Medicine, December 2018, Athens
Symposium on Lab Animal Medicine at the National Veterinary Conference, May 2018, Thessaloniki

3^rd Meeting of Hellenic Experimental Biomedical Research, Thessaloniki, Greece, Nov. 2009

1^st Meeting of Hellenic Experimental Biomedical Research, Athens, Greece, Dec. 2007

Dissemination of scientific opinions and social contribution

Seminar for the biomedicine researchers titled: “New data on the use of animal models in basic and clinical research”, 28.11.2018 at the Tsirogianni Amphitheatre, School of Veterinary Medicine, Auth, University Campus. A collaboration of the Research and Ethics Committee of the School of Veterinary Medicine with the competent authorities of Central Macedonia, Greece.

Animal welfare debate at IVSA conference, November 2016